2012 Volume 77 Issue 2 Pages 181-185
The aim of the study was to determine possible detrimental effect of nickel and zinc supplements added at various concentrations into commercial rabbit diet during 3 months of permanent feeding in an ad libitum system. The following concentrations of heavy metals were examined: P1—17.5 g NiCl2, P2—35 g NiCl2, P3—17.5 g NiCl2+30 g ZnCl2, P4—35 g NiCl2+30 g ZnCl2 to 100 kg of complete feed. Chromosome preparations were obtained from blood lymphocytes after metaphase arrest using colcemid solution. Nickel (NiCl2) presence at concentration of 35 g/100 kg of rabbit feed after 3 months of systematic feeding had a negative effect on rabbit chromosomes according to the detected aneuploidy occurence. On the other hand, ZnCl2 at concentration 30 g/100 kg of complete feed partially neutralized the negative NiCl2 effect. Significant differences in chromosomal aneuploidy were found between the P2 testing group and control (p<0.001) and between the P4 and control groups (p<0.05). In conclusion, the negative effect of selected heavy metal was clearly demonstrated through cytogenetical study based on the action of nickel element intake on an increase of aneuploidy, via a supplemented commercial rabbit diet using NiCl2 compound. Hence, it is important to point to the zinc role as a partial protector against the detrimental nickel effect on chromosomal status.