Genetic Diversity in Three Forms of Anabas testudineus Bloch

抄録

Karyotype and RAPD analysis were studied in 3 forms of climbing perch, Anabas testudineus viz. wild (native, non-spotted), Thai (introduced from Thailand, spotted) and Thai (a spotted-released form from local hatcheries). 2n=46 chromosomes were found in the spotted-released form. The total length of 2n chromosome complement was 89.96 μm with a chromosomal length ranging from 0.92 and 2.96 μm. The centromeric formula of this spotted-released form was 15m+9sm+22t. This form did not show any CMA-bands. The karyotypic features of this spotted-released form were totally different from those given by previous reports on spotted forms. Four primers, namely OPA-2, OPA-4, OPA-7 and OPA-8, were tested on these 3 forms. The wild form showed unique bands in the OPA-2 (1300, 550, 350 bp) and OPA-7 (280 bp) primers. The spotted and spotted-released form showed different RAPD banding patterns in primers OPA-4, OPA-7 and OPA-8. The wild form was found to be separate from the other 2 forms at 13.5 linkage distance. The 2 Thai forms have 11.0 linkage distances indicating that these 2 forms are not genetically very close. The karyotype and RAPD results indicate that either the Thai spotted-released form is not developed exactly from the same stock as the Thai spotted form or that due to the application of physical stress or chemical treatment this spotted-released form has been modified.