2012 年 77 巻 4 号 p. 441-446
We report the first chromosome analysis for the saddleback anemonefish (Amphiprion polymnus) from Thailand. Kidney cell samples were taken from 5 male and 5 female fish. The mitotic chromosome preparation was prepared by directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of A. polymnus was 2n=48 and the fundamental numbers (NF) was 96 in both male and female. The types of chromosomes were 6 large submetacentric, 2 large acrocentric, 10 medium metacentric, 12 medium submetacentric, 6 medium acrocentric, 10 small metacentric, and 2 small submetacentric chromosomes. The region adjacent to the telomeres on the short arms of chromosome pair 21 showed clearly observable secondary constriction/NORs. The karyotype formula for A. polymnus could be deduced as:
2n (48)=Lsm6+La2+Mm10+Msm12+Ma6+Sm10+Ssm2