2013 年 78 巻 4 号 p. 367-377
We evaluated the genome structure and salt stress response of BC1F6 lines derived from a cross between an Iranian salt tolerant bread wheat cultivar ‘Roushan’ (2n=6x=42, AABBDD) and a synthetic allohexaploid Tritipyrum (2n=6x=42, AABBEbEb). Sixteen lines (L1 to L16) and their parents were studied for their chromosome composition, K+ : Na+ ratio, and relative dry mass (RDM) by growing them in nutrient solution containing 0 (control) or 100 mM NaCl (treatment). Among the studied lines, eight wheat—Thinopyrum bessarabicum (Eb genome) disomic substitution lines were identified using PCR-based Landmark Unique Gene (PLUG) markers and cytogenetic analysis. Ten (37%) of 27 PLUG markers used were informative for Eb chromosomes 1, 2, 3, 4, and 6 in a wheat background. PLUG markers also revealed that one line, L9, was a disomic substitution line DS2Eb(2B), and that seven lines, L3, L4, L7, L8, L14, L15, and L16, were DS6Eb(6D). There were large differences in morphological traits and salinity tolerance even among DS6Eb(6D) substitutions or untranslocated lines, due perhaps to unidentified rearrangements or small introgressions.