2016 Volume 81 Issue 3 Pages 251-256
Artificial minichromosomes are exciting transgenic tools because they replicate and segregate autonomously and are not integrated into the host genome. They enable efficient delivery of an extremely large gene or multiple genes with high stability. There are two approaches to construct artificial minichromosomes: bottom-up and top-down. The construction of artificial minichromosomes addresses the basic questions of the structure and function of chromosomes. Advances in DNA synthesis technology will open a new era of generating artificial minichromosomes.