2017 年 82 巻 2 号 p. 175-181
This study aimed to investigate chlorpyrifos contamination and chromosome aberrations in H. rugulosus in an affected agricultural area compared to an unaffected area. Five H. rugulosus were collected. Chlorpyrifos concentrations were analyzed with a gas chromatograph equipped with dual capillary columns and a flame photometric detector (GC-FPD). Chromosome aberrations were studied in bone marrow. The average chlorpyrifos concentrations in H. rugulosus samples from the agricultural and unaffected areas were 11.43±7.91 and 0.31±0.31 mg kg−1, respectively. The difference in chlorpyrifos concentrations in H. rugulosus samples between the areas was significant (p＜0.05). The diploid chromosome number in H. rugulosus from both areas was 2n=26, and the numbers of chromosome aberrations in H. rugulosus in the agricultural area were higher than those in the unaffected area. There are five types of chromosome aberrations, including single chromatid gap (SCG), single chromatid break (SCB), deletion (D), centric gap (CG), and centric fragmentation (CF). The most common chromosome aberrations in the samples from the affected area were CG. The difference in chromosomal aberrations in H. rugulosus between the areas was significant (p＜0.05).