Chromosome Microdissection and Microcloning: Technique and Application in the Plant Sciences

Abstract

The technique of chromosome microdissection and microcloning, as a bridge between cytogenetics and molecular genetics, has been widely applied and play an important role in many genetic researches, such as chromosome painting probe isolation, genetic linkage map and physical map construction, and expressed sequence tags generation. To promote the further application of this technique in plants, in the present study, firstly, the experimental procedure was summarized. Secondly, the application status of this technique in plant researches was reviewed and the intractable problems of the application in plant researches were elucidated. Finally, the application of this technique in plants was looked into in the future. The results of the present study have obvious significance in promoting the application of chromosome microdissection and microcloning technique in plants.