2021 Volume 86 Issue 3 Pages 201-206
“Híbrido de Timor” (HT) ‘CIFC 4106’ is a natural allotriploid formed from Coffea arabica×C. canephora, which has been used to elucidate morphogenic in vitro responses and to regenerate new individuals, owing to its resistance to coffee pathogens and its semifertile condition. However, seedlings have not been efficiently regenerated from somatic embryogenesis hitherto. This study aimed to adapt a new indirect somatic embryogenesis procedure for HT ‘CIFC 4106,’ and to evaluate the genetic stability of plantlets regenerated. Leaf explants were inoculated in semisolid (M1) and liquid (M2) media for friable calli induction. During six subcultures (three months), the semisolid system yielded more friable calli, with visually greater cell mass. Subsequently, the friable calli of the two systems were randomly transferred to semisolid (M3) or liquid (M4) media for somatic embryo regeneration. The highest somatic embryo regeneration rate was observed in M1–M4. All recovered plantlets showed stable allotriploidy. We reinforce the relevance of adjusting in vitro conditions for indirect somatic embryogenesis establishment to provide plantlets of the different Coffea germplasms. In addition, the proposed indirect somatic embryogenesis protocol in a liquid system enables the propagation of HT ‘CIFC 4106’ plantlets, overcoming the seminal propagation barriers.
