The expression and localization patterns of BRAHMA, a core component of the SWI/SNF chromatin remodeling complex

抄録

Chromatin remodeling complexes regulate gene expression through nucleosome repositioning, thereby controlling plant development and stress responses. Among these, the SWI/SNF ATPase BRAHMA (BRM) plays a pivotal role in transcriptional regulation and hormone signaling, yet its spatial localization across organs has remained unclear. Here, we investigated the expression and subcellular localization of BRM using a translational reporter line (pBRM::BRM-GFP) in Arabidopsis thaliana. Confocal imaging revealed that BRM-GFP accumulates in the nuclei of all root cell layers, with strong enrichment at the base of lateral root primordia. In leaves, BRM-GFP was detected in mesophyll, guard, and petiole epidermal cells after tissue clearing. Co-localization with EdU staining showed that BRM distribution is independent of the cell-cycle phase or endoreduplication. These findings provide a comprehensive view of BRM localization patterns and suggest that SWI/SNF remodeling contributes to transcriptional and developmental regulation in plants.