Cytochemical Studies on Chloroplasts I

Cytologic demonstration of nucleic acids in chloroplasts

抄録

The localization of depolymerized and highly-polymerized DNA and RNA in the chloroplasts of some higher plants was demonstrated, using cytochemical methods with methyl green and pyronin and Feulgen's reaction, combined with ribonuclease digestion. The methods of the extraction of nucleic acids from the sectioned materials with trichloracetic and perchloric acids were also employed.
The author is indebted to Dr. T. Hama for valuable suggestions and advice during the course of this work and also wishes to express gratitude to Profs. Y. Mori and T. Miduno, Biological Laboratory, Keio University, for their generous help with the experiments.