Experimental Studies on the Protoplasmic Streaming in the Myxomycete Plasmodium III
The effects of amino acids and some chelating substances on the motive force of the protoplasmic streaming
1958 年 23 巻 2 号 p. 232-238
詳細
1958 年 23 巻 2 号 p. 232-238
The effects of various amino acids, ethylenediaminetetraacetic acid (EDTA) and diethyldithiocarbamate (DDC) on the motive force of protoplasmic streaming in the plasmodium, Physarum polycephalum, were investigated by means of Kamiya's double-chamber method. The following results were obtained.
1. The motive force increased with the treatment with histidine, cysteine and glycine. This increase was greatest in histidine, greater in cysteine than in glycine. These processes were reversible.
2. Other amino acids induced no marked effects on the production of the motive force.
3. EDTA inhibited the generation of the motive force to a certain extent. This inhibition was not removed by the elimination of the reagent. But, when Mg++ was admitted after the removal of EDTA, the motive force not only recovered, but rather increased as compared with its original state.
4. DDC suppressed the motive force. No marked recovery was obtained by the addition of Mg++ in this case.
5. It has been suggested that the induced increase of the motive force by amino acids may be due to the release of some metal (perphaps zinc) from the plasmodium.
