Abstract
Spermatogenesis in two millipedes Phyllogonostreptus nigrolabiatus and Gonoplectus malayus has been studied with phase contrast and cytochemical techniques. The process is the same in both species. The Golgi bodies of the early spermatid secrete acrosomal vacuoles which in turn unite to form a large proacrosome. This in turn forms the acrosome. The mitochondria react positively for tryptophan, tyrosine, phospholipides, succinic dehydrogenase and alkaline phosphatase. The Golgi bodies contain carbohydrates (1:2 glycol groups), phospholipids, tyrosine, tryptophan and acid phosphatase. The acrosomal vacuoles, the proacrosome, and the acrosome did not show positive reactions in any of the technique employed, including that of PAS.
The centriole contains carbohydrates and acid phosphatase; it is PAS positive. The mature sperm is a compressed cell with a piano-convex nucleus.
