Effect of Digitonin on Cellular Division
V. The distribution of microtubules
1973 年 38 巻 1 号 p. 55-72
詳細
1973 年 38 巻 1 号 p. 55-72
Ultrastructural studies of glutaraldehyde-osmium tetroxide fixed root tip cells of Allium sativum revealed that digitonin prevented the formation of microtubules of the mitotic spindle. In telophase, however, a large number of microtubules always formed. These phragmoplast microtubules were functional in the develop-ment of a randomly oriented cell plate. Consequently, the restituted tetraploid nucleus was always associated with a highly branched wall system. A comparative study of treated and untreated cells has indicated that the “mitotic spindle” and the “phragmoplast spindle” are two distinct and functionally independent micro-tubular organelles. Furthermore, it is suggested that the microtubular elements present in these two organelles are not entirely identical in their chemical or archi-tectural makeup.
