Localization of 5S Gene Loci on Polytene Chromosomes of Glyptotendipes barbipes (Staeger) and Transcription of RNA from These Sites

Abstract

Three loci transcribing 5S RNA have been verified on the short arm of chromosome III of G. barbipes. Evidence is also presented which indicates that a fourth site may arise on this same arm by chromosomal inversion following a break within the major 5S locus. Autoradiography after ³H-uridine incorporation indicates that the major 5S locus on chromosome III is relatively inactive in transcription in nuclei of salivary gland cells while the minor sites actively transcribe 5S RNA.