Recovery from the 5-AU Induced Blockage in Interphase: Evidence for Differential Recovery

抄録

Roots of V. faba were treated with 5-AU (350ppm) and colchicine (0.025%) in various combinations. Tetraploid cells, induced by colchicine, were used as a subpopulation of marked cells with which to study the effect of 5-AU on G₁ cells and to determine the relative rates of recovery, from the 5-AU induced block, of cells in different phases of the cell cycle. These aspects were followed in roots given 2, 18 or up to 26 hours in 5-AU: colchicine treatments were given either immediately before or after the 5-AU treatment. Cells exposed to 5-AU in the early part of interphase recovered more rapidly than those treated at the S-G₂ transition. This recovery does not appear to be due to a stimulation of cells by colchicine, since treatments with colchicine after 5-AU did not speed up the recovery of the cells. Recovery appears to be a response of the whole meristem, not just of individual cells. This suggests some form of cell-cell interaction that results in the reversal of the 5-AU blockade.