A Concurrent Ultrastructural and Ultracytochemical Study of the Peripheral Lamina in Germinal Vesicles of Pseudorhyncus japonicus (Orthoptera)

抄録

1) In the germinal vesicle of Pseudorhyncus, a peripheral lamina was found lining the inner surface of the inner nuclear membrane. It consisted of lamina members running in various directions along the surface.
2) The lamina members were digested by the action of proteolytic enzymes. This demonstrates that they are proteinaceous in chemical nature. They remained resistant against the hot perchloric acid extraction after Douglas (1970). This implies that they do not contain deoxyribonucleic acid in appreciable amount. Therefore, the lamina corresponds to the so-called “fibrous lamina” (Fawcett 1966 and others).
3) The lamina members consisted of two structurally different regions, irregular and regular. The former region exhibited a fibrous or a vesicular pattern with no definite orientation. In contrast, the latter consisted of straight cylindrical tubules or rods, lying parallel with one another and perpendicular to the lamina length. These tubules are the fundamental constituent of this region. The existence of this tubular structure in the lamina may facilitate a possible migratory movement of some small molecules between the cytoplasm and the large nucleus reinforced by the lamina.