Cytogenetical Studies on the Effect of Some Chlorinated Pesticides
II. Effect on meiotic chromosomes of Lens and Pisum
1987 Volume 52 Issue 1 Pages 55-61
Details
1987 Volume 52 Issue 1 Pages 55-61
The present study was carried out to test the mutagenic potential of some chlorinated pesticides using L. culinaris, L. esculenta, Pisum sativum and P. arvense. Studies were made through SEED-SOAK TREATMENT-pre soaked seeds were treated with different concentrations of pesticides and after thorough washing with distilled water were allowed to grow in experimental polts, and SPRAY TREATMENT-the normal plants were sprayed at flowering stage for successive 5 days. The flower buds were fixed in Farmers fixative and squashed in 1% acteto-carmine. The common abnormalities induces by all pesticides are sticky chromosomes and multivalents at metaphase-I. Laggards were frequently observed at metaphase-I but occassionally at anaphase-I and ana-telophase-II. In BHC, Lindane Heptachlor and Endrin treatment the fragments and bridges were observed in higher magnitude at anaphase-I than ana-telophase-II. Cytomixis was observed only due to spray treatment of BHC in L. esculenta L. culiuari. A few polyploid and binucleate cells were also noticed due to BHC spray treatment. Hence continuous and prolonged application of these pesticides may cause change in pure germ line.
