Histochemical Localization of Some Enzymes in Cassava Callus (Manihot esculenta Crantz.)

Abstract

Histochemical localization of two enzymes in cassava callus showing root and shootlike structures was carried out. Localization of peroxidase by the benzidine reaction showed granules in cells next to meristemoids, around root initials and tracheids and at the callus margin. They were also found close to the provascular region of the shootlike structure and parenchyma cells of its head. Phosphatase localization using the lead sulfide procedure showed black deposits in cells around the meristemoids and meristematic core of the stalk in the shootlike structure and in the vicinity of its base. They occur also in some cortical cells of the root and marginal cells of the callus.