Study of Chromosomes in two Callus Lines and in Regenerated Plants of Kallstroemia pubescens (G. Don) Dandy

抄録

The present paper analyses the variability of chromosomes in two long term callus lines of Kallstroemia pubescens (G. Don) Dandy and regenerated plantlets. The two callus lines ‘C’ and ‘F’ were distinct in morphology and growth on two different hormonal regimes. Both lines revealed an increase in the frequency of hyperdiploid mitoses with increasing age of the cultures. In long term culture, the friable ‘F’ line, as compared to the compact ‘C’, retained more diploid mitoses. A significant feature was the regular occurrence of binucleate cells in both. The causes of numerical chromosomal instabilities in vitro have been discussed. Root tip cells of the regenerated plants were diploid.