Cytomorphological Studies of Trigonella foenum-graecum Autotetraploids in Three (C₁, C₂, C₃) Generation

Abstract

Induction of autotetraploidy has been achieved in Trigonella foenum-graecum (2n=16) by cotton-swab method using 0.15% colchicine. The synthesized colchiploids (C₀) were maintained for three successive generations (C₁, C₂, C₃). Detailed morphocytological analysis has been carried out in each generation. A distinct enhancement in different vegetative parts was observed in the autotetraploids making them economically more useful. The 5 seeds collected in C₀ generation were multiplied and as many as 200 seeds were collected in C₃ generation.