Cytological Abnormalities Induced by Magnesium Sulphate in Callus Cultures of Vicia faba

Abstract

Callus tissues were established from hypocotyl segments of Vicia faba in Schenk and Hildebrandt's Medium (SH) and subcultured in modified SH media containing magnesium sulphate in different concentrations (400mg/l, 600mg/l, 800mg/l, 1000mg/l and 1200mg/l). The callus cells in primary cultures were diploid. Only rarely cells showing chromosome break or clumping of chromosomes were observed. Calli subcultured in medium containing magnesium sulphate in different concentrations showed mixoploidy, chromosome breakage and chromosome clumping.