CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Callus Induction and Plant Regeneration of Cultivated and Wild Sorghums
Jane H. GuoGeorge H. Liang
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JOURNAL FREE ACCESS

1993 Volume 58 Issue 2 Pages 203-210

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Abstract

The success of sorghum tissue culture depends upon reliable callus induction and efficient plant regeneration systems. The objective of this study was to identify callus-inducible genotypes and evaluate media for effectiveness in plant regeneration. Seven sorghum inbred lines and eight wild sorghum species were evaluated for their abilities to undergo callus induction and plant regeneration. In our research, genotype and medium effects, as well as the interaction between genotype and medium, significantly affected callus induction and plant regeneration. MA and MZ media were significantly more effective in callus induction than MN medium. MA medium was the best for plant regeneration of cultivar Xinling 1, Jinliang 5, Xin White, KS 21 R, C-401 and Pl-2, while MZ medium was the best for plant regeneration of S. versicolor, a 10 chromosome wild sorghum. The rooting medium containing NAA was the most effective in developing a strong root system. Plants regenerated from both inbred lines and wild sorghums exhibited a high degree of stability in morphology, chromosome number, and chromosome structure.

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© The Japan Mendel Society
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