Light Signals are Perceived by ATP-GTP (NTP)-binding Proteins in the Mycelia of Neurospora crassa

抄録

UV-A light (355 nm) irradiation (6μmol·m^-2·sec^-1) for I to 1000 sec of a microsomal fraction prepared from the mycelia of band and wild type strains of Neurospora crassa stimulated the binding of [α-³²P] ATP and [α-³²P] GTP to 4 NTP-binding proteins with the molecular weights, 58, 77, 83 and 129 kDa. In the former three molecules, 10^-5-10^-4 M NAD controlled the binding of [α-³²P] ATP and [α-³²P] GTP by UV-A light irradiation, and the further presence of pertussis toxin reduced the background binding of these nucleotides to these NTP-binding proteins.