Investigation on Localization of DNA in the Nucleolus-Associated Bodies of Pisum sativum L

抄録

The present study was carried out to localize DNA in the nucleolus-associated bodies (NABS) using specific staining and immunocytochemical labeling techniques in Pisum sativum L. These bodies are of interest as the functional components of plant nucleus. DNA-specific staining and labeling with anti-DNA, a monoclonal antibody, were employed to verify the presence of DNA in these bodies.
The NABs do not contain any DNA as they show negative response to DNA specific stains and also when incubated with anti-DNA, they are almost devoid of any gold particles.