Online ISSN : 1348-7019
Print ISSN : 0011-4545
Cytological Analysis of the Mature Pollen of Actinidia deliciosa (Kiwifruit)
Sachihiro MatsunagaAtsushi SakaiShigeyuki KawanoTsuneyoshi Kuroiwa
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1996 Volume 61 Issue 3 Pages 337-341


Mature pollen grains from the male and the female flowers of Actinidia deliciosa (kiwifruit) were examined by DAPI (4', 6-diamidino-2-phenylindole) -fluorescence microscopy. The generative nucleus and the vegetative nucleus were observed in the male pollen grain while neither nucleus was observed in the female pollen grain. In the generative cell of the pollen grains from the male flower, many organelle nuclei were observed. The DNA content of the generative and the vegetative nuclei was measured fluorimetrically by propidium iodide staining with a video-intensified microscope photon counting system. The DNA content of the generative nucleus was about twice as large as that of the vegetative nucleus. This suggests that the generative nucleus has 2C DNA and is arrested in the G2 phase.

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© The Japan Mendel Society
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