1997 年 62 巻 3 号 p. 303-308
We investigated the best fixation methods for the immunogold electron microscopy. Rapidfreeze substitution is better than conventional chemical fixation for preservation of structural integrity of cell nucleus, mitochondrion and plastid. When samples were embedded in LR White resin, fixation in absolute acetone gave a 2.6-fold increase in immuno-labelling of DNA compared to fixation with 1% glutaraldehyde in acetone. In contrast, no gold particles were identified when cells were fixed by 1% OsO4 and embedded in Spurr's resin. These results indicate that the rapidfreeze substitution in absolute acetone and embedding in LR White resin is the optimal approach for immunogold labelling.
