Quantitative Analysis of Transcripts from Two Zygote-specific zysl Genes in Chlamydomonas reinhardtii

抄録

Primer extension analysis using 10 μg of polyA RNA extracted from zygotes of Chlamydomonas reinhardtii 10 min after mating was carried out to elucidate relative amounts of transcripts of early zygote-specific (zys1) genes. A novel minor extension product as well as a major product for each of the zys1A and zys1B genes was detected. Quantification of band intensity showed that relative accumulation amounts of zys1A major, zys1A minor, zys1B major and zys1 minor transcripts were in the ratio of 1.78 0.171 : 1.00 : 0.730. This result suggests that the zys1A promoter is slightly stronger than that of zys1B at an initial stage of gene expression.