Colchicine-Induced Autotetraploid in Aloe vera L.
2001 年 66 巻 4 号 p. 409-413
詳細
2001 年 66 巻 4 号 p. 409-413
Autotetraploid plants of Aloe vera L. were induced by colchicine treatment. Diploid shoot tips (2n=2x=14) were treated by immersion of the rhizomes in colchicine concentrations of 0.05%, 0.10% and 0.15% at exposure times of 6, 12, 18 and 24 h. The highest proportion of polyploid plants (5.9%) with 2n=4x=28 was obtained from a 0.15% colchicine and 24 h combination. Diploid/tetraploid chimerical plants were observed at the lowest colchicine concentration/time treatments. Expression of gigantism in morphological and cytological characters, such as, width and thickness of the leaves, size and frequency of juvenile foliar spots, plant weight, size of epidermal and root cells, were noted in autotetraploid plants with a significant decrease in stomatal frequency.
