造血器腫瘍の免疫学的診断の標準化成人白血病における細胞表面マーカー検査の標準化：JCCLSガイドライン提示

抄録

Flow-cytometry (FCM) of cell surface markers is routinely used for the clinical diagnosis of hematopoietic malignancies in our institution. This test is important for the diagnosis and for the planning of treatment schedule but, presently, we face with the problem of loss (absence) of standardization of the laboratory procedures. Similar to internal quality control, the external quality control is essential to guarantee the compatibility of data at an inter-institutional level. However, at present, there is no basic manual, and therefore, no standardization. “Guidelines for performing surface antigen analysis on hematopoietic malignant cells.” (JCCLS H2-A V1.0) was extracted from FCM-WG (Chairperson: Shiro Miwa) of the Japanese Committee for Clinical Laboratory Standards (JCCLS) for the guarantee of precision, accuracy and standardization in 2003. The present report aims to introduce the “JCCLS H2-P V1.0 guideline” as an example of standardization for the tests for the analysis of hematopoietic tumor cell surface markers in adult leukemia.