2006 年 16 巻 2 号 p. 43-49
The stathomokineses method was presented as the cell cycle analysis to determine cell cycle parameters. In this method, all of the relationships are derived from an equation that n(t, τ) =αN0exp(αt)exp(ατ), where n(t, τ) is cell density of a phase τ at time t, N0 is the initial cell number at time t = 0, Tc is cell-cycle time and α = ln2 / Tc. The stathomokinetic agents, colchicines or demecolcine (Colcemid), are added in a cell population to block cell cycle progression at the M phase. The plotting of G1 or G2/M cell fractions against time reveals the cell cycle parameters. A computer program of cell cycle analysis of unconstrained-type is presented to help the stathomokinetic analysis.