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Online ISSN : 2424-0664
Print ISSN : 0916-6920
ISSN-L : 2424-0664
総説
フローサイトメトリ-を用いた細胞周期解析Ⅲ
― スタトモキネティック解析 ―
藤川 孝三郎宮越 稔
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ジャーナル フリー

2006 年 16 巻 2 号 p. 43-49

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The stathomokineses method was presented as the cell cycle analysis to determine cell cycle parameters. In this method, all of the relationships are derived from an equation that n(t, τ) =αN0exp(αt)exp(ατ), where n(t, τ) is cell density of a phase τ at time t, N0 is the initial cell number at time t = 0, Tc is cell-cycle time and α = ln2 / Tc. The stathomokinetic agents, colchicines or demecolcine (Colcemid), are added in a cell population to block cell cycle progression at the M phase. The plotting of G1 or G2/M cell fractions against time reveals the cell cycle parameters. A computer program of cell cycle analysis of unconstrained-type is presented to help the stathomokinetic analysis.

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