微量細胞対応型オンチップセルソーターシステムの開発

抄録

We have developed a series of single-cell-based analysis called ‘on-chip cellomics', consisting of singlecell-based sorting for purifying target cells, single-cell-based cultivation on chip for fully geometric/temporal control of target cells, and single-cell-based genome/proteome analysis for identifying target biomarkers of epigenetic phenomenon. For ‘on-chip cellomics' study, non-destructive cell sorting is the most important for the following re-cultivation of target cells on the cultivation chips keeping their intact condition and information. We thus have proposed and examined four approaches of non-destructive cell purification; aptamermagnet microbeads method exploiting digestable DNA/RNA-based aptamer probes, apoptosis method using spot irradiation of focused laser beam to promote apoptosis on cells except for target cells, removable fluorescent probe detection method with disposable microchip, and ultra high-speed camera image detection method with disposable microchip. The results indicated those four methods are non-destructive and can be used for the sufficient selective cell purification for the following on-chip cell cultivation. In this paper, we introduce briefly about our results and potential of those cell purification methods and compare the advantages and defects of those methods.