2015 年 25 巻 2 号 p. 27-32
Patient’s donor specific antibody (DSA) prior to transplantation is at a high risk of developing antibody mediated rejection (AMR) and show a lower rate of graft survival after transplantation. It is thought that graft failure can be prevented by early identification and removal of clinically harmful DSAs associated with AMR. In this study, we focused on and investigated the influences of factors affecting the results of flow cytometry crossmatch (FCXM) and of the types of measuring instruments used. As a requirement for investigation of the inter-instrument difference, it was decided that measurement should be performed at the same time on the same day. Measurement was performed after preparing the samples by washing once to 3 times after the primary antibody response, and adding the secondary antibody by the usual method. The correlation of the ratios between Canto and Calibur was investigated by using channel values, and a positive correlation was found; the correlation coefficient for T cells was R2 = 0.8237 and that for B cells was R2 = 0.8709. We performed analysis by changing the measurement method to the one using the channel values. As a result, a positive correlation was found. Molecules of equivalent soluble fluorochrome (MESF) was also measured by changing the method to that using the channel values, and a calibration curve was prepared. With the change of the measurement method, the calibration curve crossed with the longitudinal axis, which allowed lower values to be detected with a higher sensitivity.