Journal of Environment and Safety
Online ISSN : 2186-3725
Print ISSN : 1884-4375
ISSN-L : 1884-4375
Topics & Reports
Acute toxicity test of ZnCl2 with or without NaCl or NaH2PO4 using medaka larvae: its effect on mortality and cell proliferation
Mayuka OhiraGento NakagawaMasafumi OhtaYasuhiro IshibashiKoji ArizonoSeiji YokoyamaHotaka KaiMasahiro Yamaguchi
Author information

2015 Volume 6 Issue 1 Pages 31-42


Zinc is one of the industrially important metals widely used as materials of such as plating and brass. In addition,zinc is one of the essential minerals which is necessary to keep human body healthy. On the other hand, intake of an excess amount of zinc would be toxic for organisms including human, thus the environmental standard is prescribed in Japan. In this study, we examined toxic effect of zinc chloride on medaka larvae. We found that LC50 of zinc on medaka larvae was 5.4 (5.2-5.7 95% CL) mg/L. Furthermore, we showed that sodium chloride and sodium dihydrogenphosphate reduced the toxicity, especially the effect of sodium dihydrogenphosphate was remarkable. Histological and proliferation defect were not detected even in the zinc-treated larvae. These results confirmed the fact that co-existing sodium salt affects the toxicity of zinc, and the effect varies depending on forms of the salts.In addition, it was revealed that the defects of the cells caused by zinc in culture system does not necessarily arise in living organisms treated with zinc.

Information related to the author
© 2015 Academic Consociation of Environmental Safety and Waste Management,Japan
Previous article