2003 Volume 113 Issue 7 Pages 1095-1104
In order to identify novel target genes for therapeutic agents and diagnostic markers for atopic dermatitis (AD), we utilized the cDNA array to search for genes whose expression is altered in AD skin. We analyzed the gene expression of 548 genes, including inflammatory genes and those that are responsible for the skin barrier, in the skin of AD patients with or without concomitant allergic respiratory diseases and in that of healthy volunteers. We found that, of the 548 probe genes on the membrane, the expression levels of 58 genes differed between AD patients without concomitant allergic respiratory diseases and healthy volunteers. Among these 58 genes, the differential expression of 30 genes had been reported previously, but the differential expression of the other 28 genes were identified in this study for the first time. Moreover, we revealed that these 58 genes could discriminate AD patients from healthy volunteers, regardless of the presence of concomitant allergic respiratory disease. Our results indicate that systematic gene expression analyses are useful tools for detecting the novel target genes for therapeutic agents and diagnostic markers for AD.