Article ID: 2023-010
Posterior capsule opacification is a common complication following cataract surgery. However, its incidence varies depending on the type of intraocular lens (IOL) used. In this study, we analyzed the adhesion and movement of lens epithelial cells (LECs) in the early stages after cataract surgery by continuously observing LECs seeded on the surfaces of different types of IOLs. PY-60AD and XY1 hydrophobic acrylic IOLs were used. Each posterior surface was modified to increase the adhesion. The IOLs were placed in culture dishes with an exposed posterior or anterior surface (three each). LECs extracted from white rabbit lenses by trypsinization were seeded onto IOLs. LEC movement and shape were recorded by microscopy using a cell culture observation system after 6 h of contact between LECs and IOLs. The cumulative distance traveled was calculated from the LEC trajectories after comparing the shapes of the LECs. We observed that LECs were more likely to adhere to XY1 modified posterior surface. The speed of LEC movement decreased after adhesion. These findings support the conclusion that increasing the adhesiveness of IOL surfaces inhibits the LEC movement.
