A Study of a Simple Giardia Detection Method Using Diluted Sternheimer-Malbin Stain Solution for Urine Sediment Stain

Abstract

Giardia is a potentially zoonotic parasite; nevertheless tests for detecting Giardia are not commonly performed in small animal clinics, because of difficulty of the fecal test, the need of sufficient skills for the cyst test, and the lack of information about Giardia. Therefore, we contrived a simpler method to detect cysts of Giardia by negative staining with urinary stain solution (Sternheim-Malbin Stain Solution) diluted three times with normal saline. In comparison with enzyme linked immunosolvent assay (ELISA), this simple technique is valuable as a screening test for Giaradia cysts.