高圧濾紙電気泳動法によるアミノ酸の分離定量 (第5報)

中性アミノ酸の分離について

抄録

Of the analysis method for the neutral amino acids, two dimensional chromatography is very commonly used.
But, this method is not so convenient, for it takes a long time to obtain the results. In our recent reports, the investigation was made on the determination of basic amino acids; lysine, arginine, histidine, by using highvoltage-paper electrophoresis method.
In this paper, owing to its advantage, we tried to use highvoltage-paper electrophoresis on one side of the two dimensional chromatography for the separation of neutral amino acids so as to shorten the time requiring for this procedure.
As the results of the experiment, a clear diagram of separated 11 neutral amino acids, except leucine and iso-leucine was obtained with in a half period of usual procedure.