Electrochemistry
Online ISSN : 2186-2451
Print ISSN : 1344-3542
ISSN-L : 1344-3542
Communications
A Screening of Phage Display Library for a Neutral Saccharide, ‘Chitin’ in a Medium Containing Ethanol
Eiichiro FUKUSAKITsuyoshi NAKANISHIKouichi OGAWAAkio KOBAYASHI
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JOURNALS OPEN ACCESS

2001 Volume 69 Issue 12 Pages 966-968

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Abstract

It is difficult to isolate peptide ligands that bind to saccharides from phage display random peptide library since an interaction between peptides and saccharides is likely to be restricted to hydrogen bond. We performed a phage peptide library screening against a neutral polysaccharide, ‘chitin’ in the media consisting of organic solvent to reduce non-specific hydrophobic interaction and to emphasize hydrogen bond. As a result, the phage library converged to the specific clones that bind to a constituent sugar unit of chitin.

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© 2001 The Electrochemical Society of Japan
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