Electrochemistry
Online ISSN : 2186-2451
Print ISSN : 1344-3542
ISSN-L : 1344-3542
Communications
Fructosyl Amine Sensing Based on Prussian Blue Modified Enzyme Electrode
Wakako TSUGAWAKinuko OGAWAFumimasa ISHIMURAKoji SODE
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JOURNALS OPEN ACCESS

2001 Volume 69 Issue 12 Pages 973-975

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Abstract

Low potential amperometric enzyme sensor for fructosyl-valine, a model compound of glycated hemoglobin (HbA1c), was constructed using a fructosyl-amine oxidase (FAO) and Prussian blue film as the artificial peroxidase. The Prussian blue-based FAO electrode exhibits a good linear correlation between 0.1 to 0.3 mM, similar to what was observed with the low potential enzyme electrode employing FAO, peroxidase, and ferrocene as the electron mediator. These results indicated that the Prussian blue-based FAO electrode may avoid the inherent problems of hydrogen peroxide measurement at the high potential and also have advantage in its simple construct of the system compared with the two enzyme peroxidase - FAO - ferrocene system.

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© 2001 The Electrochemical Society of Japan
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