2001 Volume 69 Issue 12 Pages 984-986
The electrochemical properties of oxidase-peroxidase co-immobilized electrode fabricated electrochemically deposited polyaniline (PAn) were investigated. It found that electro-conductivity of a PAn film was necessary for the oxidase HRP to show its function. Based on this result, the enzyme switch utilizing the oxidase-HRP system was developed by utilizing electro-conductivity changes of a PAn film. It was found that a PAn film lost its electro-conductivity by the oxidase-HRP reactions, and the oxidized PAn film obtained electro-conductivity again by applying + 100 mV vs. Ag/AgCl to a PAn film.