Electrochemistry
Online ISSN : 2186-2451
Print ISSN : 1344-3542
ISSN-L : 1344-3542
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Electric Gene Expression in Single-cells of Rice Protoplast via Ca2+ Entering the Cell
Hideaki MATSUOKATamu KOMAZAKIYoshiko MUKAIMikako SAITO
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2008 Volume 76 Issue 8 Pages 625-630

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Abstract

Spatial distribution of intracellular Ca2+ concentration was measured with a spectro-imaging system composed of an image slicer (10×10 channels), a grism, and a high sensitive CCD camera. The Ca2+ concentration of each single protoplast was different from others both at a steady state (10–100 nmol dm−3) and after electric stimulation. Therefore traceable observation of each single-cell was essential, which contrasted conventional methods dealing with an average of many cells. When a pulsing electric stimulation was applied to single-cells of rice protoplast, the transient variation of intracellular Ca2+ concentration was observed and chitinase gene expression followed (16 out of 36 cells, 44.4%). Its significance level was estimated as 90% by χ2-test.

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© 2008 The Electrochemical Society of Japan
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