2009 Volume 77 Issue 4 Pages 319-321
A glucose-sensing electrode based on the cathodic detection of oxygen, which was consumed with the glucose oxidase-catalyzed reaction in the presence of glucose, was prepared by immobilizing the enzyme on a surface-modified poly(dimethylsiloxane) (PDMS) layer. A PDMS layer was prepared on a platinum electrode by casting an emulsion of the polymer, the polymer surface was treated with oxygen plasma to replace silane groups with silanol groups, and then a GOx layer was prepared on the PDMS layer by applying silanization and cross-linking chemistries. The oxygen plasma-treatment of PDMS did not cause significant changes in the permselectivity with the polymer layer so as to discriminate hydrophilic solutes, such as hydrogen peroxide, from oxygen. Thus, the resulting electrode could be used for the measurement for glucose (0.02–1.8 mM) without the error caused by L-ascorbic acid and uric acid.
