Electrochemistry
Online ISSN : 2186-2451
Print ISSN : 1344-3542
ISSN-L : 1344-3542
コミュニケーション
Electrochemical Characterization of Enzyme and Immunoglobulin G Patterned Using Microcontact Printing
Hitoshi SHIKUAyako KUMAGAIHong Qun LUOYasufumi TAKAHASHITomoyuki YASUKAWAHiroshi YAMADATomokazu MATSUE
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2010 年 78 巻 2 号 p. 122-125

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Microcontact printing (μCP) with various proteins has been widely applied to biosensors and cell biology research. However, the mechanism of protein patterning in the μCP process is not clear in detail. We have electrochemically estimated enzyme concentration on glass slide patterned with μCP technique. We also estimated the enzyme concentration at the surface of poly(dimethylsiloxane) (PDMS), which corresponded to the enzyme activity at the PDMS stamp just before μCP. Our result suggested that it was possible to transfer enzyme monolayer from PDMS to glass surfaces with 100% efficiency with μCP. Immunoglobulin G (IgG) patterned with μCP was also characterized by tagging with enzyme labeled anti-IgG. Scanning electrochemical microscopy (SECM) image was obtained to visualize line and space pattern of IgG monolayer.

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© 2010 The Electrochemical Society of Japan
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