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Electrochemistry
Vol. 84 (2016) No. 5 p. 338-341

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http://doi.org/10.5796/electrochemistry.84.338

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This paper describes a method of hairpin DNA (hpDNA) unzipping analysis using a biological nanopore array. Various lengths of hpDNAs were captured and translocated through the nanopore and the individual duration times were monitored. The correlation between the unzipping time and the simulated hybridization energy of the duplexes was able to be modeled as first-ordered reaction. This method is one of the approach for understanding of basic biological reactions in DNA replication and RNA transcription.

Copyright © 2016 The Electrochemical Society of Japan

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