Rapid Fabrication of Nanoporous Gold as a Suitable Platform for the Direct Electron Transfer-type Bioelectrocatalysis of Bilirubin Oxidase

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Nanoporous gold fabricated via anodization in a buffer solution comprising potassium chloride served as an effective scaffold for the direct electron transfer-type bioelectrocatalysis of bilirubin oxidase from Myrothecium verrucaria (BOD). BOD adsorbed on a porous gold electrode showed an increased population of effective orientations required for direct electron transfer reactions. The catalytic current of BOD reached an oxygen-transfer-limited value at the nanoporous gold electrodes despite neutral conditions.