Investigating the Blocking Effect of Alkanethiol Self-assembled Monolayer on Electrochemical Response of the Split Gq-based DNA-NTs-based Biosensor

Abstract

The split G-quadruplex-based DNA-nanotweezwers-based electrochemical DNA sensor was characterized by focusing on the blocking ability of the mixed self-assembled monolayer (SAM) against the nonspecific adsorption of hemin and the direct reduction of oxygen on a gold electrode. We found that the mixed SAM composed of MCH and MHA (concentration of each alkanethiol in SAM formation solution was 1.0 mM : 0.1 mM) effectively suppressed the nonspecific adsorption of hemin and the direct reduction of oxygen on the gold electrode.