Electrochemistry
Online ISSN : 2186-2451
Print ISSN : 1344-3542
ISSN-L : 1344-3542
Significance of Nano-Structures of Carbon Materials for Direct-Electron-Transfer-type Bioelectrocatalysis of Bilirubin Oxidase
Mizue WANIBUCHIYui TAKAHASHIYuki KITAZUMIOsamu SHIRAIKenji KANO
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JOURNALS OPEN ACCESS Advance online publication
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Article ID: 20-64063

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Abstract

Three types of widely used carbon materials were examined as scaffolds for the direct-electron-transfer (DET)-type bioelectrocatalysis of bilirubin oxidase (BOD) as an electrocatalyst for a 4-electron reduction of oxygen (O2). The carbon materials used were: Ketjen Black EC300J (KB) with a primary particle size (ϕp) of ca. 40 nm and a hollow structure, Vulcan XC-72R (Vulcan) with ϕp of 37 nm and a filled structure, and high purity graphite SP series (JSP) with ϕp of 10 µm and well-developed micropore structures. For the three carbon materials, the rotating disk steady-state limiting catalytic current density of the O2-reduction (|jc,lim|) increased with the non-Faradaic current (|jb|) at small |jb| values and was saturated at large |jb| values. The |jc,lim/jb| ratio in low |jb| range was in the following order: JSP ≫ Vulcan > KB. Electrochemical and microscopic data suggested that microporous structures of JSP are highly effective for the DET-type reaction of BOD. Gaps between several primary particles in the KB and Vulcan aggregates play important roles as scaffolds for BOD. The inner surface of partially broken KB particles is electrochemically active to give large |jb| but not effective as BOD scaffolds.

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© The Author(s) 2020. Published by ECSJ.

This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License (CC BY, http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse of the work in any medium provided the original work is properly cited. [DOI: 10.5796/electrochemistry.20-64063].
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