2021 Volume 65 Issue 2 Pages 51-62
Phos-tag electrophoresis, which was developed by Kinoshita et al. in 2006, has attracted the attention of many researchers as an epoch-making method that can analyze the phosphorylation state of proteins. The authors were involved in the research and development of Phos-tag diagonal electrophoresis as one of the new techniques for Phos-tag electrophoresis. In this review, the authors describe why research was initiated to develop Phos-tag diagonal electrophoresis, and proteomic significance and the practical protocol of this electrophoresis.
