Electrophoresis Letters
Online ISSN : 2189-2636
Print ISSN : 2189-2628
ISSN-L : 2189-2636
Development of a fully automated enzymatic lipoprotein fractionation method for staining total cholesterol and triglycerides
Makoto MatsushitaNatsumi YamaguchiMarina Tanaka
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2021 Volume 65 Issue 2 Pages 69-73

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Abstract

For lipid staining of lipoprotein fractions on electrophoresis, Fat red 7B and Sudan black B are used. These dyes utilize the lipid-soluble characteristic of lipophilic dyes. Accordingly, the value of the fraction may vary due to differences in lipids contained in lipoproteins. We have recently developed a new fully automatic lipoprotein fractionation method, the CH•TG method, which stains total cholesterol (CH) and triglycerides (TG) using enzymes. Compared with the conventional Fat red method, the separation of LDL and VLDL became clearer by the CH•TG method, and full automation was possible. The correlation with the Fat red method was y=1.04x−9.1, r=0.934 for the HDL fraction, y=1.27x−6.6, r=0.893 for the VLDL fraction, and y=1.26x−3.2, r=0.734 for the LDL fraction. In addition, the VLDL and LDL fractions by the CH•TG method better reflected fluctuations in TG and CH, respectively. Based on these results, the CH•TG method may be a new lipoprotein fractionation method that overcomes the problems with the Fat red method.

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© 2021 Japanese Electrophoresis Society
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