Abstract
To ascertain, whether insulin might have mutual relatibn against adrenalin on the nitrogen metabolism, these experiment was carried out in the nine healthy femal dogs.
Each dog kept in the usual metabolic cage war fed upon a fixed diet, one group on carbohydrate-rich and the other on carbohydrate-poor.
The urine obtained by catheterisation at 9. a. m. was mixed with that collected in the reciever of the cage, and diluted to a constant volume.
The total nitrogen was estimated by Kjeldahl's method urea by plimmer-Skelton's method, ammonia, creatinine and creatin by Folin's method ; sugar by Pavy-Kumagawa-Sudo's method ; aceton and acetoacetic acid by Messinger-Huppert's method.
As soon as the animals were in nitrogenous equilibrium, the adrenalin solution was injected subcutaneously and a week after the above dose of adrenalin were given with the subcutaneous injection of insulin again.
The influence both of the adrenalin and adrenalin-insulin on the urine component was observed.
In order to avoid the error caused by the so-called epinephrin habit, at first the injection of the adrenalin-insulin and next of the adrenalin alone was tried.
The results of the experiment Were summerized as follows.
I) The effects in the case of the carbohydrate-rich diet.
a) The administration of adrenalin caused the marked increase in the excretion of total-and creatin-nitrogen and acetonbodies followed by the appearance of sugar in the urine, but no changes in the output of urea-, ammonia-and creatinine-nitrogen.
b) The output of total-, ammonia-, creatin-nitrogen, sugar, and acetonbodies in the urine was less increased by the adrenalin-insulin-injection than the adrenalin alone, but no change was in the excretion of urea-and creatinine-nitrogen in both cases.
II) The effect in the case of the carbohydrate-poor diet.
a) The administration of the adrenalin produced the increase in the excretion of tatol-, ammonia-, creatin-nitrogen and the appearance of acetonbodies with sugar, but no remarkable change in the output of creatinine-and urea-nitrogen.
b) The output of tatol-, ammonia-, creatin-nitrogeri, sugar and acetonbodies was less increased by the adrenalin-insulin-injection than the adrenalin alone, but no change in the output of the creatinine-and urea-nitrogen.
III) From the above-mentioned data, it can be concluded that the insulin acts to restrain the increase in the output of total nitrogen, other nitrogen components, sugar and acetonbodies, which were produced by the adrenalin.
